Crystallin promoter

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WebJul 15, 1992 · Progeny from two of these lines were mated with transgenic mice expressing the Cre recombinase under control of either the murine alpha A-crystallin promoter or … WebJul 1, 2002 · It is proposed that Pax6 occupies and represses the chicken βB1-crystallin promoter in lens epithelial cells, and is displaced by Prox1 and Mafs, which activate the promoter, in differentiating cortical fiber cells. ... It is shown that the transactivation potential of c-Maf and MafB for the rat gammaD-crystallin Maf-responsive element (gammaD ...

Transcriptional Regulation of the Mouse αA-Crystallin Gene: …

WebPromoter methylation of RASEF was associated with increased risk of death due to metastasis, ... Epigenetic regulation of αA-crystallin in high myopia-induced dark nuclear cataract. PLoS One 2013; 8: e81900. doi: 10.1371/journal.pone.0081900. 8. Mao W, Rubin JS, Anoruo N, Wordinger RJ, Clark AF. WebFGF2 strongly activates expression of the αA-crystallin gene promoter coupled with the DCR1 enhancer compared to the promoter alone. 234 Interestingly, FGF signaling also regulates expression of a large set of miRNAs that modulate gene expression during FGF2-induced lens fiber cell differentiation. 157 At least three miRNAs, including miR-143 ... the paint room

The zebrafish as a model system for analyzing mammalian and native

WebDec 1, 2012 · In many crystallin gene promoters, elements for lens and non-lens expression are combined in the same 5 ′ region. However, one of the more recently … WebSox-1 transactivated the γF-crystallin promoter in a dose-dependent fashion, similar to c-Maf (Fig. 2 C). Sox-1 also stimulated the βB2 promoter, but less strongly than c-Maf (Fig. 2 B). Prox-1 (80 ng) stimulated the βB2- and γF-crystallin promoters only about 2-fold (Fig. 2, B and C). To determine whether these transcription factors can ... WebWe have further compared the γD-crystallin promoter with the γB-crystallin promoter, the promoter with the most extended developmental expression. We show that the γ … the paint room connellsville pa

Insertion of a Pax6 consensus binding site into the alphaA …

The zebrafish as a model system for analyzing mammalian and …

WebA DCR1/αA-crystallin promoter (including DCR2) coupled with EGFP virtually recapitulated the expression pattern of αA-crystallin in lens epithelium and fibers. In contrast, the DCR3/αA/EGFP reporter was expressed only in 'late' lens fibers. Chromatin immunoprecipitations showed binding of Pax6 to DCR1 and the αA-crystallin promoter … WebMar 21, 2024 · Recruits the transcriptional coactivators CREBBP and/or EP300 to crystallin promoters leading to up-regulation of crystallin gene during lens fiber cell … the paint room connellsville pa menuWebCrestline serves sophisticated institutions, family offices and high-net-worth individuals in North America, Europe and Asia who seek innovative alternative investment solutions … the paint school

"WebEarlier experiments showed that the betaB1-crystallin promoter sequence from -120 to -48 contains a cis element (PL2 at -90 to -76) that stimulates the activity of a heterologous promoter in lens cells but not in fibroblasts. " - Crystallin promoter

Crystallin promoter

WebFeb 21, 2024 · The predicted promoter probability and location, and the type of the mutation are shown above each sequence (TSS at position 100). b–d, TERT promoter point mutations predicted by the CNN model... WebSep 1, 2002 · Thus, the chicken betaB1-crystallin promoter directs high level RNA expression to lens fiber cells, which may be especially useful for the expression of ribozyme and anti-sense RNAs in addition to ...

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WebI am a highly motivated and passionate individual with a talent for organization and project management. I have enjoyed leading teams to complete projects in various industries … WebMar 1, 2003 · In order to study lens-retina relationships during development, we cloned the zebrafish alphaA-crystallin cDNA and its promoter region. Using a 2.8-kb fragment of the zebrafish alphaA-crystallin promoter (z (alpha)Acry), we expressed the diphtheria toxin A fragment (DTA) in zebrafish embryos in a lens-specific manner.

WebThe two paralogous zebrafish αB-crystallin promoters produced subtly different expression profiles, with the aBa promoter driving expression equally in notochord and skeletal muscle while the αBb promoter resulted primarily in skeletal muscle expression. WebJan 15, 2000 · Many of the crystallin promoters have been carefully dissected into component cis -acting elements with critical regions and putative transcription factor binding sites identified ( Cvekl and Piatigorsky, 1996; Duncan et al., 1996; Matsuo et al., 1991 ).

WebDec 3, 2013 · CpG island methylation in the αA-crystallin promoter was similar in age-related cataract and high-myopic cataract classified as Lens Opacity Classification System III Grade Nuclear Color 2–3. Pyrosequencing, a real-time DNA sequencing method, was used to determine single-base variations caused by CpG methylation of the CRYAA … WebMay 4, 2006 · Dramatic upregulation of αA-crystallin expression is associated with primary lens fiber cell differentiation. Previous studies have shown that a −366 to +46 αA …

WebThe human papillomavirus type 16 (HPV-16) E6 and E7 oncogenes are thought to play a role in the development of most human cervical cancers. These E6 and E7 oncoproteins affect cell growth control at least in part through their association with and

WebLens-specific expression and developmental regulation of the bacterial chloramphenicol acetyltransferase gene driven by the murine alpha A-crystallin promoter in transgenic … shutterfly download all photosWebMar 1, 1989 · Recently Landel et al. (1988) have combined the α -crystallin promoter with the ricin cytotoxic gene to produce a single line of transgenic mice with a somewhat more severe phenotype consisting of a still smaller lens and other interesting eye malformations. shutterfly download all photos to pcWebCrystallins are a diverse group of abundant soluble proteins that are responsible for the refractive properties of the transparent eye lens. We showed previously that Pax-6 can activate the αB-crystallin/small heat shock protein promoter via the lens-specific regulatory regions LSR1 (−147/−118) and LSR2 (−78/−46). shutterfly download pcWebMar 30, 2024 · Stimulation of αA-crystallin promoter activity via 8-bromo-cAMP, forskolin, or human T-cell leukemia virus type I Tax1 in transfections and reduction of activity of this site in cell-free transcription tests by competition with the somatostatin CRE supported the idea that DE1 is a functional CRE. Finally, Pax-6, a member of the paired-box ... shutterfly discounts 2023WebThe same SOX proteins also regulate γ-crystallin genes. the mammalian counterpart of δ crystallin. A conserved and essential DNA motif in the promoters of the γ-crystallin genes is the binding site of the SOX proteins that activate the promoters (Kamachi et al., 1995). SOX1 strongly, and SOX2 and SOX3 modestly, activate these crystallin ... the paint room connellsvilleWebNov 3, 2003 · In order to identify PaxB response elements in the J3-crystallin promoter, we generated a series of J3 promoter deletions in the luciferase reporter construct. The shortest promoter fragment, consisting of only 200 bp upstream of the transcriptional start site, was activated by PaxB and not by PaxB(I84R) (data not shown; but cf. Figure 5C ) . the paint shack eau claire wiWebApr 8, 2024 · Among the 14 crystallin genes that human and mouse have in common, mRNA levels of Crybb1, Crygd, Cryba2, Cryba4, Crybb3, Cryga, and Crygs were significantly up-regulated in myopic eyes of... shutterfly download full resolution