Ph-ncas9-ppe
Webb27 feb. 2024 · Examination of 92 pH-nCas9-PBE-transformed plants revealed that 40 harbored at least one C to T substitution in the target region (mutation efficiency of 43.48%) ( Fig. 2d and Supplementary... http://www.solentcgcom.com/139674/
Ph-ncas9-ppe
Did you know?
Webb15 apr. 2024 · To construct the pH-nCas9 (H840A) vector for the control group, the M-MLV sequence of pH-nCas9-PPE was removed using a ClonExpress II Cloning Kit. The PE3 … WebbPlasmid pH-nCas9-PPE from Dr. Caixia Gao's lab contains the insert nCas9(H840A)-M-MLV and is published in Nature Biotechnology This plasmid is available through Addgene. Addgene: pH-nCas9-PPE - yobet手机,yobetag娱乐城,yobet网上投注
WebbPlasmid pH-nCas9-PPE-V2 from Dr. Caixia Gao's lab contains the insert nCas9(H840A)-M-MLV and is published in Nat Biotechnol. 2024 Mar 25. pii: 10.1038/s41587-021-00868-w. … WebbLin Q, Zong Y, Xue C, Wang S, Jin S, Zhu Z, et al.Prime genome editing in rice and wheat. Nat Biotechnol. 2024;38:582-585 pubmed publisher
WebbpH-nCas9-PPE-V2 Plasmid #170131 Purpose For plant prime editing in rice plants or monocotyledons protoplasts Depositor Caixia Gao Article Lin et al Nat Biotechnol. 2024 Mar 25. pii: 10.103 Insert nCas9 (H840A)-M-MLV Use CRISPR Tags Expression Plant Mutation H840A for Cas9; D200N, T306K, W313F, T330P and L… Promoter maize … WebbFor sgRNA expression in monocotyledons protoplasts. Loading... 140447. pH-nCas9-PPE. For plant prime editing in rice plants or monocotyledons protoplasts. Loading... 140445. …
WebbThis plasmid was modified from pKDL071 described in Litcofsky K.D., Afeyan R.B., Krom R.J., Khalil A.S., Collins J.J.; Iterative plug-and-play methodology for ...
Webb7 jan. 2024 · product information. Strains containing DP6 must be maintained in rich media supplemented with 25 mM glucose. Received from Ahmed Badran, former Liu lab member. how did the ancient chinese make paperWebbPlasmid pCre-AntiDsRed from Dr. James Birchler's lab contains the insert AntiDsRed Cre recombinase and is published in Unpublished This plasmid is available through Addgene. how did the ancient chinese use paperWebbpOsU3 pTaU3 pH-nCas9-PPE-V2/pH-ePPE Forward primer (5'-3'): + Spacer sequence + Reverse primer (5'-3'): + PBS and RT template sequence + Submit >> clear. Help document. There are 35458 Visits, latest from 52.167.144.144 ... how many squares are in a charm packhttp://www.plantgenomeediting.net/ how did the ancient egyptians communicateWebb18 dec. 2024 · A fragment containing these two sgRNA expression cassettes was PCR amplified and cloned into the HindIII-digested pH-nCas9-PBE binary plasmid (Fig. S1b). All recombinant plasmids were subjected to DNA sequencing for verification. Primers used for molecular cloning are listed in Table S1. Generation of transgenic plants how did the ancient egyptians make breadhttp://www.biofeng.com/adjy/zhili/pH-nCas9-PBE.html how many squares are on a halma game boardWebb27 feb. 2024 · We used a CRISPR-Cas9 nickase-cytidine deaminase fusion to achieve targeted conversion of cytosine to thymine from position 3 to 9 within the protospacer in both protoplasts and regenerated rice,... how did the ancient egyptians use papyrus