Phos stat3
WebMar 6, 2007 · Phosphosphorylated STAT3 is known to dimerize and translocate from the cytoplasm to the nucleus before binding to the promoter of its target genes. To examine the effect of PTPRT on the subcellular localization of STAT3, we examined the cells described in the above experiment with immunofluorescence. WebCell line description: HEK-Blue™ IL-6 cells were generated by stable transfection of human embryonic kidney 293 (HEK293)‑derived cells with the genes encoding human IL-6 receptor (IL-6R) and signal transducer and activator of transcription 3 (STAT3). In order to detect the activation of the IL-6 pathway, they were further transfected with a ...
Phos stat3
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WebSTAT3 is an 88 kD member of the STAT (signal transducer and activators of transcription) protein family that is phosphorylated in response to a cytokine receptor-associated … WebApr 1, 2024 · Depending on the identity of STAT3 and its effect on regulatory networks, dephosphorylation of STAT3 by protein tyrosine phosphatase can occur in either the cytoplasm or the nucleus. To reveal the dephosphorylation of STAT3 by the PTPN14, we performed an IFA in Vero cells. In order to capture the dephosphorylation reaction of …
WebAug 18, 2024 · The pcDNA3.1-HK2/STAT3 include Neomycin resistance gene as a known selectable marker of stable mammalian transfectants and β-lactamase (the Ampicillin resistance gene) as the selectable marker in the properly transformed prokaryotic hosts, and eucaryote expression vector pcDNA3.1 (+) and target products were digested with NheI … WebMar 6, 2007 · STAT3 Is a Substrate of PTPRT. To identify potential substrates of PPTRT, we generated two ecdysone-inducible HEK293T cell lines: one expressing the intracellular …
WebDec 1, 2015 · Although STAT3-associated kinases such as JAK, Abl and PKC have been characterized 12, 13, relatively little is known about the nuclear phosphatases that modulate the transcriptional activity of... WebSpecific antibodies included CCL2 (#710,002, Thermo Fisher Scientific Inc., Waltham, MA, USA), Janus kinase 2 (JAK2, ab108596, Abcam Inc., Cambridge, UK), phos-JAK2 (phospho Y1007 + Y1008, ab32101, Abcam), signal transducer and activator of transcription 3 (STAT3, phospho Tyr705, GTX104616, GeneTex, Inc., Alton Pkwy Irvine, CA, USA), phos-STAT3 …
WebMay 3, 2005 · STAT3, DNMT1, and histone deacetylase 1 form complexes and bind to the SHP-1 promoter in vivo. Treatment with pharmacologic grade DNMT1 anti-sense oligonucleotides and STAT3 small-interfering RNA induces in the malignant T cells DNA demethylation and expression of SHP-1gene.
WebNational Center for Biotechnology Information how do i cite google earthWebSTAT3-induced PI3K/Akt signaling negatively regulates PTEN/TLR4 pathways in LPS-stimulated BMM in vitro. (A/B) Western-assisted expression of phos-STAT3, Akt, PTEN, … how do i cite myself as a sourceWebAug 25, 2015 · Patient has unequivocal pathologic diagnosis of AML (≥ 20% blasts in the bone marrow based on World Health Organization (WHO) criteria), excluding acute promyelocytic leukemia t (15;17) (q22;q12); Promyelocytic leukemia gene (PML)- retinoic acid receptor alpha (RARA) Age ≥ 65 years old No prior treatment for AML except: … how do i cite in apa formatWebThe Src homology 2 (SH2) domain-containing protein tyrosine phosphatase 1 (SHP-1), a non-receptor protein tyrosine phosphatase, has been reported as a negative regulator of phosphorylated signal transducer and activator of transcription 3 (STAT3) and linked to tumor development. In this present review, we will discuss the importance and function of … how do i cite in a paragraphWebFeb 25, 2024 · Expectedly, both mRNA and protein levels of hexokinase-2 (HK2) and PKM2 were increased in colon epithelial cells following 7-day DSS treatment, accompanying with the induction of pho-STAT3 and c-Myc ( Fig. 1 E and F ). how do i cite in a paperWebFeb 16, 2016 · Gabrilovich and colleagues demonstrate that tumor monocytic cells rapidly differentiate to macrophages due to hypoxia inducible decrease of STAT3 activity. Inhibition of CD45 phosphatase mediated by sialic acid was responsible for this effect. Degradation of sialic acid sensitized tumor myeloid cells to STAT3 inhibitor, resulting in potent antitumor … how do i cite myself in apaWebMetrics. Author & Article Info. Abstract. AIMS/HYPOTHESIS: T cell protein tyrosine phosphatase (TCPTP, encoded by PTPN2) regulates cytokine-induced pancreatic beta cell apoptosis and may contribute to the pathogenesis of type 1 diabetes. However, the role of TCPTP in pancreatic endocrine function and insulin secretion remains largely unknown. how do i cite from a website