WebMay 20, 2005 · The GFP-syp puncta colocalize with a CFP-actin dendrite; 2. The maximal intensity of the puncta is at least half of the maximal intensity of the brightest puncta from the same cell; and 3. The area of the puncta (edge defined as half peak intensity) is at least 0.6 μm 2 (20 pixels when using 40× objective). Synaptic density for an axonal ... WebJun 2, 2024 · We counted all SYP-immunoreactive puncta that had a spherical appearance with clear boundaries. Examples of stained puncta are indicated with arrowheads. Scale …
What is the meaning of "synaptic puncta"? - ResearchGate
WebTwo-photon time-lapse observations followed by quantitative analysis of tagged SYP levels at individual synapses demonstrated the time course of synaptogenesis: increases in … WebOct 2, 2014 · Cells were immunostained with antibodies against GFP, SYP, and PSD95. PN axons were visualized by GFP, which is presented in white pseudocolor. The arrowheads indicate SYP puncta that are closely apposed with postsynaptic PSD95 puncta. The arrows indicate PSD95-free SYP puncta. The yellow arrow and arrowhead indicate the magnified … established vending route business for sale
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WebJul 17, 2015 · Dotted line represents the total number of SYP (+) puncta of Ctrl 0.8. ( c and d) Colocalization of FM (+) terminals following 5AP- and 600AP-stimulation at the same branches ( c ). 51.3 ± 9.8 % (n = 12 coverslips, totally 7542 5AP-induced puncta and 14701 600AP-induced puncta were analyzed) and 90.6 ± 2.9 % (n = 16 coverslips, totally 16926 … WebMar 7, 2024 · Immunostaining for the presynaptic marker SYP and postsynaptic marker PSD95 in mCherry – hCOs fused with mCherry + hThOs revealed that mCherry + axons in hCOs either contained PSD95 + puncta adjacent to neighboring local SYP + puncta (Figure 4 H, panel a), or contained SYP + puncta adjacent to local PSD95 + puncta (Figure 4 H, … WebNov 26, 2024 · We found that 14 days after ischemic stroke, astrocytes and microglia/macrophages engulfed synaptic elements immunolabeled for both presynaptic (synaptophysin, SYP) and postsynaptic (Homer-1)... firebase new project